Fig. 1

CU06-1004 reduces H₂O₂ -induced inhibition of HBMEC growth. A Chemical structure of CU06-1004. B Human brain microvascular endothelial cells (HBMECs) were incubated with increasing concentrations of CU06-1004 (1, 2, 5, 10, 20 µg/ml) for 48 h. Cell viability was determined using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. C Cell viability of HBMECs treated with increasing concentrations of H₂O₂ for 24 h. Cell viability was determined using MTT assay. D HBMECs were pretreated with CU06-1004 (5–20 µg/ml) for 1 h before 100 µM H₂O₂ exposure. After 24 h, cell viability was determined using MTT assay. All data were analyzed with one-way analysis of variance followed by Tukey’s multiple comparisons test. B-C. ***P < 0.001 vs. control. n.s., not significant. D. ^###P < 0.001 vs. control. ***P < 0.001 vs. H₂O₂. Results are presented as the mean, and error bars represent the standard error of the mean