Fig. 1
From: Apicobasal transferrin receptor localization and trafficking in brain capillary endothelial cells
In vitro BBB model integrity. Schematic illustration of the applied non-contact co-culture (NCC) in vitro blood–brain barrier (BBB) model setup including primary porcine brain endothelial cells (pBECs) and astrocytes (a), with validation of the barrier integrity by transendothelial electrical resistance (TEER) (Ω cm2) presented as mean values (± SEM) and vertical line marking the day of inducement (b), and confocal microscopic imaging with 60 × magnification of immunofluorescent stainings (c) visualizing the tight- and adherens junctional (TJ and AJ) proteins claudin-5, ZO-1, occludin and p120 catenin (red), and Hoechst stain of nuclei (blue), with scale bar equal to 10 μm