Fig. 6

Differential gene expression analysis of subtype of microglia in brain tissue post-FUS BBBD. (A) Heat map of microglia-specific transcripts. The heat map showing the clustering of M1 and M2 genes in response to FUS-BBBD (0.25 MPa and 0.42 MPa). The DEGs based on log₂FC values were divided into pan microglia (black), M1-specific (red), and M2-specific (green) transcripts in the RNA sequence data set. Blue and red indicate a decrease and increase in expression compared with the sham control, respectively. (B) Gene expression correlation between qRT-PCR and RNA-seq data CD68, Aif1, and Ccl12 are pan-microglia-specific genes. Sepine1, CD40, and Gbp4 are M1-specific genes. Arg1, Gpr84, and Apod are M2-specific genes. The light blue (0.25 MPa) and dark blue (0.42 MPa) columns represent the qRT-PCR results (left y-axis). The red lines indicate the FPKM values (right y-axis). The error bars show the standard deviation (STDEV) for the replicates in each experiment. *P < 0.05, **P < 0.01, ***P < 0.001 (two-tailed Student’s t-test; vs. Sham)