Fig. 6

Tocilizumab reduces expression of IL-6 in macrophages in the lesion epicenter after SCI. A, B Representative images of the spinal cords of the sham, PBS, and Tocilizumab groups are stained for IL-6 (green) and F4/80 (red) at 14 (A) and 28 dpi (B), which exhibits most colocalization of F4/80 and IL-6 in the injured core in the PBS group, while less colocalization of F4/80 and IL-6 in the Tocilizumab group. Asterisks indicate the injured core. Scale bar: 100 μm. B Quantification of the IL6⁺ macrophages at 14 and 28 dpi. Data are presented as the mean ± SEM (n = 5 in each group). ****p < 0.0001 (Tocilizumab vs. PBS). ^####p < 0.0001 (sham vs. PBS and Tocilizumab)