Fig. 2

Laminin receptors mediate the vascular pattern of intracerebrally injected laminins. A Representative images of Alexa-555 labeled laminin-111 (red) in the brain at 24 h after intracerebral injection in the presence of IgM control, ITGB1 function-blocking antibody, DAG1 function-blocking antibody, and both ITGB1 and DAG1 function-blocking antibodies. B Quantification of Alexa-555 fluorescent intensity in blood vessels in A. n = 4, *p = 0.0286 by Mann–Whitney U test. C Representative images of Alexa-555 labeled laminin-111 (red) in the brain at 24 h after intracerebral injection in the presence of CaCl₂ or EDTA. D Quantification of Alexa-555 fluorescent intensity in blood vessels in C. n = 4, *p = 0.0286 by Mann–Whitney U test. Data are represented as mean ± SD. DAG1, dystroglycan; ITGB1, integrin-β1; EDTA, ethylenediaminetetraacetic acid