Fig. 2
From: The choroid plexus: a door between the blood and the brain for tissue-type plasminogen activator
CPECs uptake tPA ex vivo. Freshly isolated CP explants were treated for 15 min with tPA and BSA (40 nM each), alternatively coupled to Alexa555 or Alexa488. A Dose-dependent fluorescent signal generated by tPA555 (magenta) and BSA488 (green) after SDS-PAGE. B Representative confocal photomicrographs of CP explants exposed 15 min to 40 nM of tPA555 (magenta), BSA488 (green) and DAPI staining (white). Scale bar 40 µm and 30 µm in the magnified inserts. C Graph shows quantification of fluorescence in CP explants (n = 5, mean ± SEM), * Significant difference (p < 0.05). D Dose-dependent fluorescent signal generated by tPA488 (green) and BSA555 (magenta) after SDS-PAGE. E Representative confocal photomicrographs of CP explants exposed 15 min to 40 nM of tPA488 (green), BSA555 (magenta) and DAPI staining (white). Scale bar 40 µm and 30 µm in the magnified inserts. F Graph shows quantification of fluorescence in CP explants (n = 5, mean ± SEM), * Significant difference (p < 0.05)