Table 2 primers of Tet 2 siRNA and hMeDIP-qPCR
From: ROS attenuates TET2-dependent ZO-1 epigenetic expression in cerebral vascular endothelial cells
Tet2 siRNA-1 | F: 5′-CCCAGAGUCCUAAUCCAUCUATT-3′ R: 5′-UAGAUGGAUUAGGACUCUGGGTT-3′ |
Tet2 siRNA-2 | F: 5′-AAGCUAGCGUCUGGUGAAGAATT-3′ R: 5′-UUCUUCACCAGACGCCUAGCUUTT-3′ |
Tet2 siRNA-3 | F: 5′-CAAGGCAGUGCUAAUGCGUAATT-3′ R: 5′-UUAGGCAUUAGCACUGCCUUGTT-3′ |
SiRNA NC | F: 5′-UUCUCCGAACGUGUCACGUTT-3′ R: 5′-ACGUGACACGUUCGGAGAATT-3′ |
Homo Tjp1-CpG1- Homo Tjp1-CpG- | F: 5′-ATAAGTAATACAGCATGAAA-3′ R: 5′-TGCTGGCCGGGAGCTGCTGT-3′ |
Homo Tjp1-CpG2 | F: 5′-AGCTATGCACCTGCCCAGTA-3′ R: 5′-TCCCTCGCTGGCGGCTGTCT-3′ |
Homo Tjp1-CpG3 | F: 5′-CAAATAAACATCTCCCGAGA-3′ R: 5′-AGTGGGTGGCCGCGCTCGGA-3′ |
Homo Tjp1-CpG4 | F: 5′-CCTAGAGACCTGTGACATCT-3′ R: 5′-AACAAGAATTTCAGCAATCTT-3′ |