Fig. 3
From: Microglia-derived CCL2 has a prime role in neocortex neuroinflammation
Representative images of neocortex sections from naïve (a), EAE-affected (b; cs 3.5), and EAE-affected MSC-treated (c; cs 2.25) mice, sacrificed 24 h after MSC treatment, and double immunostained for NG2 and CCL2. a NG2-glia is recognizable (arrowheads) also associated to the microvessel wall (V); note an NG2-reactive pericyte (arrow) and the CCL2 staining of the microvessel (V). b, c NG2 is not colocalized with CCL2 on the activated NG2-glia (arrowheads), characterized by increased branching and NG2 upregulation, whereas the chemokine appears restricted to NG2− macrophage/microglia-like ramified cells (arrows). TOPRO-3 nuclear counterstaining. Scale bars: a–c 25 μm