Fig. 5
From: Activation of NMDA receptors in brain endothelial cells increases transcellular permeability
Activation of NMDA receptor induces rapid dislocalization of markers for the BBB at the capillary level in the mouse cortex. A–D Activation of NMDA receptor induces rearrangement of the brain endothelial cells and pericytes in a receptor specific manner. C57/BL6 mouse was injected with PBS (A), NMDA (0.5 mg/kg) (B), NMDA with MK801 (0.5 mg/kg each) (C), and MK801 (0.5 mg/kg) (D) for 1 min and mice were sacrificed. Brain sections were stained with CD31 (Red, 1:500), GFAP (Green, 1:500), and CD13 (Gray, 1:500), a marker for endothelial cells, astrocytes, and pericytes, respectively. Magnified images of each treatment are displayed at the bottom panel. Arrow indicates the CD31 positive lamellipodia like structure. Arrowhead indicates where devoid of CD13 signal is observed at the brain vasculature is observed. Scale bar indicates 200 μm and 25 μm for upper and bottom panel, respectively. For NMDA treated mouse (B) Scale bar indicates, 200 μm (top panel), 25 μm (middle panel), 5 μm (bottom panel)