Fig. 4
From: Activation of NMDA receptors in brain endothelial cells increases transcellular permeability
Activation of NMDA receptor induces rapid modulation of Rho-GTPase and actin cytoskeletal reorganization (A) Activation of NMDA receptor induces upregulation of the Rho-GTPase activity and was depicted as a graph (n = 3). C Inhibition of Rho associated kinase by Y27362 (0.5 μM) in NMDA treated brain endothelial cells decreases in Rab5 (Green), and enhances LAMP1 (Red). Scale bar indicates 75 and 25 μm for upper and bottom panel, respectively. Images from upper panel is enlarged and presented at the bottom panel. D Inhibition of PKC by Staurosporine (2 nM) induces decreases in Rab5 (Green) and enhances LAMP1 (Red) compared to its NMDA only control. Images from upper panel is enlarged and presented at the bottom panel. Scale bar indicates 75 and 25 μm for upper and bottom panel, respectively