Fig. 6

Upregulation of the choroidal immune machinery in PHH rats and NKCC1 hyperactivity. a RNAseq of choroid plexus from PHH and control rats revealed transcription of 13,884 genes. Of the transcripts detected at ≥ 0.5 TPM, 5944 were considered differentially transcribed. A total of 1302 transcripts were linked to inflammation, 92 encoded immune receptors, while the remaining 1210 encoded other inflammatory agents involved in mediating immune responses. b Schematic illustration of those inflammatory markers found elevated in the CSF from PHH patients that also displayed upregulated transcription of their corresponding receptors (or co-receptors) in the rat choroid plexus after IVH. c Choroidal ⁸⁶Rb⁺ efflux after 16 h incubation in absence (control, n = 5) or presence (n = 5) of a mix of inflammatory markers (CCL3, OSM, IL-10 and IL-6, all at 500 ng/ml), with the efflux rate constants plotted in d, e Choroidal ⁸⁶Rb⁺ influx after 16 h incubation in absence (control, n = 5) or presence (n = 5) of a mix of inflammatory markers (CCL3, OSM, IL-10 and IL-6, all at 500 ng/ml). Error bars represent standard deviation and statistical significance was tested with an unpaired two-tailed t-test. *P < 0.05, NS not significant