Table 3 Tissue origin of extracellular vesicles and techniques used for labeling
From: Extracellular vesicles through the blood–brain barrier: a review
Author | Organ of origin | Cell line | Labeling | Result |
|---|---|---|---|---|
Morad et al | Breast cancer | MDA-MB-231 | Gaussia luciferase; TdTomato | EVs crossed monolayer |
Tominaga et al | Breast cancer | MDA-MB-231-luc-D3H1 and MDA-MB-231-luc-D3H2LN | PKH67; PKH26; DiR | Detected in endothelium but not pericytes or astrocytes |
Chen et al | Embryonic kidney | HEK 293 T cells | Gaussia luciferase fused with lactadherin; PKH67; PKH26 | Crossed only with TNF-α treatment |
Matsumoto et al | Parkinson´s disease and healthy control RBC | Human erythrocytes | Na125I; Na131I; Dil | Crossed only with LPS treatment |
Kuroda et al | Melanoma | SK-Mel-28 | PKH67 | Successful incorporation but not crossing of the endothelium |