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Fig. 4 | Fluids and Barriers of the CNS

Fig. 4

From: Exposure of human cerebral microvascular endothelial cells hCMEC/D3 to laminar shear stress induces vascular protective responses

Fig. 4

Impact of shear stress exposure on NRF2 protein localization. Left: cells were seeded in Ibidi microslides and cultured either in static condition or under a shear stress of 10 dyn.cm−2 for 72 h. Staining was performed with an antibody against NRF2, phalloidin and dapi. Shown are representive images of 14 images taken in random locations. Right: quantification of the mean fluorescence intensity of NRF2 in the nucleus and the cytosol was performed using Image J analysis on 14 images per condition (~ 50 cells per image). Results are presented as Nuclear/Cytosol (N/C) ratio in percentage of the control (static) condition

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Fluids and Barriers of the CNS

ISSN: 2045-8118