Fig. 10

Protein levels of transporters and receptor in hCMEC/D3 cells cultured either in static or under shear stress exposure of 5 or 10 dyn.cm⁻². Data are presented as means \(\pm\)standard deviation (a) Label free quantification (n = 4 to 5) (b) Targeted proteomic analysis of ABC transporters from two independent experiments in triplicates. Expression ratio are normalized with the corresponding value in static. c Functionality of P-gp and MRP transporters. Calcein-AM or DCFDA were respectively incubated for 30 min with or without specific inhibitor (respectively Valspodar or MK-571). Histograms present the cellular uptake of fluorescent metabolites of substrates normalized with the value static condition without inhibitor. Three independent experiments were performed in triplicate. Statistical significance was determined by ANOVA followed by a Tukey’s test (*p < 0.05; **p < 0.01; ***p < 0.001)