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Fig. 3 | Fluids and Barriers of the CNS

Fig. 3

From: Ptpn20 deletion in H-Tx rats enhances phosphorylation of the NKCC1 cotransporter in the choroid plexus: an evidence of genetic risk for hydrocephalus in an experimental study

Fig. 3

Generation of Ptpn20−/− mice. A Schematic illustration of Ptpn20 locus. Ptpn20 gene was targeted at exon 4. The sgRNA sequence is underlined in yellow. The protospacer adjacent motif sequence is indicated in the red dotted box. Electropherograms of the 104-bp deletion mutations in exon 4 are indicated in the black dotted box (sgRNA = single guide RNA). B Expression levels of Ptpn20 protein in CP were verified by immunoblotting. The 50-kDa bands correspond to Ptpn20 and nonspecific bands. Ptpn20 protein shows almost completely absence in the CP of Ptpn20−/− mice. Actin is used as the loading control (n = 5 mice per group). C Immunofluorescence of Ptpn20 (green) and F-actin (red) in the CP of 8-week-old WT and Ptpn20−/− mice. Ptpn20 is almost completely absent in the CP of Ptpn20−/− mice, while no significant difference is evident for F-actin compared to WT. Areas in square frames are magnified in the bottom panels. Top panels, scale bar = 50 μm; bottom panels, scale bar = 20 μm. D Representative transmission electron microscope images of longitudinal sections of lateral ventricle CP from 8-week-old WT and Ptpn20−/− mice. No structural difference in CP is evident between WT and Ptpn20−/− mice. Scale bar = 20 μm

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Fluids and Barriers of the CNS

ISSN: 2045-8118