Fig. 6

Rab7 decorates α-syn carriers. Quantification of Rab7 and VPS35 co-localization with ɑ-syn added from the luminal or abluminal side. a, b are micrographs of pBEC cells with luminally and abluminally added ɑ-syn, respectively, co-stained for Rab7. d, e are micrographs of pBEC cells with luminally and abluminally added ɑ-syn, respectively, co-stained for VPS35. Representative dual-color confocal micrograph (i) with Rab7 or VPS35 (red) and ɑ-syn (green), scale bar show 5 µm. Maximum z-projected 3D STED stack (ii) of the highlighted area in (i), scale bar show 1 µm. Micrographs (zx-i) and (zx-ii) are sideview representations of the cells from maximum y-projections of the 400 nm slices highlighted in (ii). Scale bar show 1 µm. All images are contrast adjusted to enhance clarity. Pearson’s correlation coefficients between Rab7 and ɑ-syn are shown in (c) and between VPS35 and ɑ-syn in (f)