Fig. 5

Effect of intracellular trafficking machinery on α-syn transport through the BBB model. Representative micrographs of α-syn uptake in pBEC’s on filters knocked down for VPS35 and Rab7 and treated for 2 h with monomeric α -syn, as indicated in (a). Scale bars show 15 µm. Relative percentage of α-syn transcytosis in cells pretreated with indicated siRNA (in comparison to scrambled siRNA) and treated for 2 h with α-syn from luminal (b) and abluminal (c) side of the BBB model, respectively. Bar plots in (b, c) show mean values of ELISA measurements. Statistical difference was tested using an ordinary one-way ANOVA followed by Dunnett's multiple comparisons test. Representative Western blot to confirm the efficiency of the siRNA mediated knockdown of indicated proteins in pBECs on Transwell filters