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Fig. 1 | Fluids and Barriers of the CNS

Fig. 1

From: Processing mechanism of guanidinoacetate in choroid plexus epithelial cells: conversion of guanidinoacetate to creatine via guanidinoacetate N-methyltransferase and monocarboxylate transporter 12-mediated creatine release into the CSF

Fig. 1

Localization of monocarboxylate transporter 12 (MCT12) in the rat choroid plexus. AC Double immunohistochemical staining of MCT12 (red) and Na+, K+-ATPase (green) in the rat choroid plexus. Na+, K+-ATPase is used as a marker of the apical membrane of choroid plexus epithelial cells (CPxEpic). Arrowheads indicate the apical membrane of CPxEpic. D and E Immunohistochemical staining of anti-MCT12 antibodies after antigen absorption. Anti-MCT12 antibodies were incubated in PBS (−) with or without antigenic peptides of anti-MCT12 antibodies (4.36 µg/mL) for 6 h at 4 °C and then used as the primary antibodies. Nuclei were stained with 4ʹ,6-diamidino-2-phenylindole dihydrochloride (DAPI, blue). Scale bar: 50 µm. F Protein expression of MCT12 in CPxEpic. Anti-MCT12 antibodies were incubated in blocking buffer with or without antigenic peptides of anti-MCT12 antibodies (4.36 µg/mL) for 6 h at 4 °C and then used as the primary antibodies

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