Fig. 1

Localization of monocarboxylate transporter 12 (MCT12) in the rat choroid plexus. A–C Double immunohistochemical staining of MCT12 (red) and Na⁺, K⁺-ATPase (green) in the rat choroid plexus. Na⁺, K⁺-ATPase is used as a marker of the apical membrane of choroid plexus epithelial cells (CPxEpic). Arrowheads indicate the apical membrane of CPxEpic. D and E Immunohistochemical staining of anti-MCT12 antibodies after antigen absorption. Anti-MCT12 antibodies were incubated in PBS (−) with or without antigenic peptides of anti-MCT12 antibodies (4.36 µg/mL) for 6 h at 4 °C and then used as the primary antibodies. Nuclei were stained with 4ʹ,6-diamidino-2-phenylindole dihydrochloride (DAPI, blue). Scale bar: 50 µm. F Protein expression of MCT12 in CPxEpic. Anti-MCT12 antibodies were incubated in blocking buffer with or without antigenic peptides of anti-MCT12 antibodies (4.36 µg/mL) for 6 h at 4 °C and then used as the primary antibodies