Fig. 6

Oxidative stress modulates iBMEC turnover and immune cell adhesion. a Representative images of the time course of cell turnover events over an 18-min period. b–d Quantification of mitosis rate, apoptosis rate, and turnover, respectively, in response to vehicle, acute oxidative stress, and chronic oxidative stress treatments at day 1 after exposure. n = 5 biological replicates for control, n = 3 biological replicates for chronic and acute exposure. e Representative images of microvessels four days after initial exposure to vehicle, acute, and chronic oxidative stress, where fluorescent THP-1 (magenta) adhesion is indicated by magenta arrows and delaminations are indicated by white arrows. Note that adhered THP-1 cells are completely absent in the vehicle condition. f Quantification of adhered THP-1s at days 1 and 4 after initial exposure to oxidative stress. g Characterized counts of adherent THP-1 cells according to their distance from a delamination across H₂O₂ exposures (up to 150 µm “upstream” of delamination, up to 150 µm “downstream” of delamination, or “unassociated”). Data f–g represents n = 218 adherent cells across all conditions with n = 3 independent microvessels for each condition