Fig. 1From: Effects of acute and chronic oxidative stress on the blood–brain barrier in 2D and 3D in vitro modelsMethods to model oxidative stress in 2D and 3D. a Schematic depicting experimental protocol of exposing 2D iBMEC monolayers on Transwells to chronic and acute doses of H2O2. b Schematic depicting seeding of iBMECs into 3D microchannels, formation of BBB microvessels, and exposure to chronic and acute H2O2Back to article page