Fig. 7
Effects of l-ornithine (l-orn, 20 mM, 24 h) on the general oxidative state of cultured primary brain endothelial cells. a Reactive oxygen species production measurement using the DCFDA reagent. In this assay hydrogen peroxide (H2O2, 100 µM) was used to induce oxidative stress. n = 5–12. b Nitric oxide (NO) production was detected by using the DAF-FM diacetate reagent. In this assay sodium nitroprusside (SNP, 100 µM) was used as NO donor. n = 8. c Mitochondrial network continuity calculation using Matlab software. Continuous signal lengths were measured and divided by all object number detected to result in the average length of objects. n = 9–19. Cyanide agent (CCCP, 5 µM) was used to decouple mitochondrial networks. d Representative pictures of the mitochondrial network staining using the Mitotracker green labeling. Blue: cell nucleus. Bar: 10 µm. Control groups (C) received only culture medium. On all graphs, values are presented as means ± SEM. Statistical analysis: one-way Anova followed by Bonferroni post-test, *, p < 0.05; ***, p < 0.001