Table 1 Antibodies
Target antigen | Vendor | Catalog number | Dilution |
|---|---|---|---|
5-Bromo-2'-deoxyuridine (BrdU) | abcam | ab6326 | 1:250 |
PFKFB3 | Cell Signaling | 13123S | 1:500 |
GLUT1 | EMD Millipore | 07-1401 | 1:10,000 |
HK2 | Cell Signaling | 2867 T | 1:1000 |
MCT1 | Proteintech | 20139-1-AP | 1:10,000 |
CLDN5 | ThermoFisher Scientific | 35-2500 | 1:50 |
OCLN | ThermoFisher Scientific | 33-1500 | 1:50 |
ZO-1 | ThermoFisher Scientific | 61-7300 | 1:25 |
PECAM-1 | Sigma | P8590 | 1:25 |
VE-Cadherin | R&D Systems | AF938 | 1:25 |
\(\beta\)-actin \(\beta\)-actin, HRP-linked | Cell Signaling abcam | 3700S ab49900 | 1:10,000 1:20,000 |
Anti-mouse IgG, HRP-linked | Jackson ImmunoResearch | 115-035-003 | 1:5000 |
Anti-rabbit IgG, HRP-linked | Jackson ImmunoResearch | 111-035-144 | 1:5000 |
AlexaFluor® 488-Anti-rat IgG | ThermoFisher Scientific | A-21208 | 1:500 |
AlexaFluor® 488-Anti-mouse IgG | Jackson ImmunoResearch | 715-545-150 | 1:200 |
AlexaFluor® 594-Anti-rabbit IgG | Jackson ImmunoResearch | 711-585-152 | 1:200 |
AlexaFluor® 594-Anti-goat IgG | Jackson ImmunoResearch | 705-585-147 | 1:200 |