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Table 1 Antibodies

From: Prolonged culturing of iPSC-derived brain endothelial-like cells is associated with quiescence, downregulation of glycolysis, and resistance to disruption by an Alzheimer’s brain milieu

Target antigen Vendor Catalog number Dilution
5-Bromo-2'-deoxyuridine (BrdU) abcam ab6326 1:250
PFKFB3 Cell Signaling 13123S 1:500
GLUT1 EMD Millipore 07-1401 1:10,000
HK2 Cell Signaling 2867 T 1:1000
MCT1 Proteintech 20139-1-AP 1:10,000
CLDN5 ThermoFisher Scientific 35-2500 1:50
OCLN ThermoFisher Scientific 33-1500 1:50
ZO-1 ThermoFisher Scientific 61-7300 1:25
PECAM-1 Sigma P8590 1:25
VE-Cadherin R&D Systems AF938 1:25
\(\beta\)-actin
\(\beta\)-actin, HRP-linked
Cell Signaling
abcam
3700S
ab49900
1:10,000
1:20,000
Anti-mouse IgG, HRP-linked Jackson ImmunoResearch 115-035-003 1:5000
Anti-rabbit IgG, HRP-linked Jackson ImmunoResearch 111-035-144 1:5000
AlexaFluor® 488-Anti-rat IgG ThermoFisher Scientific A-21208 1:500
AlexaFluor® 488-Anti-mouse IgG Jackson ImmunoResearch 715-545-150 1:200
AlexaFluor® 594-Anti-rabbit IgG Jackson ImmunoResearch 711-585-152 1:200
AlexaFluor® 594-Anti-goat IgG Jackson ImmunoResearch 705-585-147 1:200