Fig. 6

Activation of Rac1 with Rac1 CRISPR Activation (CRISPRa) abolished the beneficial effects of Met-R on neurological deficits and BBB disruption 24 h after ICH mice. A Modified Garcia test. B Forelimb placement test. C Corner turn test. D Representative western blot bands. E–K Quantitative analyses of CCR1, p-SRC, Rac1, albumin, MMP9, claudin-5, occludin, and ZO-1 in the ipsilateral hemisphere at 24 h after ICH. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 vs. sham, ^#p < 0.05 ^##p < 0.01, ^###p < 0.001 vs. ICH + vehicle, and ^@p < 0.05 ^@@p < 0.01, ^@@@p < 0.001 vs. ICH + Met-R + Scr CRISPR. Data were expressed as mean ± SD. n = 6 per group