Table 1 Medium for neuronal expansion and differentiation
From: Modeling ischemic stroke in a triculture neurovascular unit on-a-chip
Reagent | Supplier | Catalogue number | Final concentration |
|---|---|---|---|
N2B27 medium | |||
Neurobasal medium | Gibco | 21103049 | Â |
N2 supplement | Thermo Fisher | 17502-048 | 1× |
B27 supplement | Gibco | 12587-010 | 1× |
Non-essential amino acids | Gibco | 11140050 | 1% |
GlutaMAXâ„¢ supplement | Gibco | 35050038 | 1% |
Penicillin-streptomycin | Sigma | P4333 | 1% |
2-mercaptoethanol | Gibco | 31350-010 | 50 µM |
Expansion medium | |||
|---|---|---|---|
N2B27 medium | Â | Â | Â |
EGF | Sigma | E9644 | 10Â ng/mL |
FGF | PeproTech | 100-18B | 10Â ng/mL |
Differentiation medium | |||
|---|---|---|---|
N2B27 medium | Â | Â | Â |
BDNF | PeproTech | 450–02 | 20 ng/mL |
GDNF | PeproTech | 450–10 | 10 ng/mL |
Ascorbic acid | Sigma | A4544 | 100 µM |
db-cAMP | Sigma | D0627 | 10 µM |