Fig. 3

The inflammatory markers CCL28, CCL23, and CCL3 do not modulate the NKCC1 activity. a Schematic illustration of the inflammatory markers in cocktail 1 (CCL28, CCL23, and CCL3) and their corresponding receptors. b ⁸⁶Rb⁺ efflux from the rat choroid plexus as a function of time in the control setting (black, n = 4) or with exposure to cocktail 1 (green, n = 4) containing CCL28 (100 ng/ml), CCL23 (200 ng/ml), and CCL3 (100 ng/ml). The y-axis represents the natural logarithm of the ⁸⁶Rb⁺ amount left in the choroid plexus at time T (A_T) divided by the initial amount at time 0 (A₀). c ⁸⁶Rb⁺ efflux rate constant in the control setting (0.80 ± 0.09 min⁻¹, n = 4) or with exposure to cocktail 1 (0.70 ± 0.12 min⁻¹, n = 4, CCL28 (100 ng/ml), CCL23 (200 ng/ml), and CCL3 (100 ng/ml). P = 0.21. d ⁸⁶Rb⁺ efflux rate constant in the control setting (0.82 ± 0.11 min⁻¹, n = 4) or with exposure to high concentrations of cocktail 1 (0.80 ± 0.19 min⁻¹, n = 4, CCL28 (500 ng/ml), CCL23 (500 ng/ml), and CCL3 (500 ng/ml), P = 0.86. Error bars in b, c and d represent standard deviation and statistical significance was tested with an unpaired two-tailed t-test. NS, not significant