Fig. 7
From: Tachycardia and hypertension enhance tracer efflux from the spinal cord
Fluorescent ovalbumin (AFO-647) injected into the spinal interstitium labels blood vessels and central canal. a, b, d In both extramedullary and intramedullary arteries and arterioles, tracer deposited distinctly in multiple layers (confocal microscopy, × 100 magnification). AFO-647 was found external to, and within the tunica media (marked by *) of the anterior spinal artery, a, grey matter arteriole, d, and arterial vasocorona, b. Arrows mark the yellow endothelium, while the layers of green AFO-647 are marked by oppositely pointing sets of arrow heads and arrows. d Note the delineation of neuronal nuclei and the tortuous spinal extracellular space by AFO-647. c, e The central canal at × 100 magnification on confocal microscopy. The left arrow head in e and the up arrow head in c emphasise the heterogeneous, speckled deposition of tracer favouring the luminal aspect of the ependymal lining (* is the lumen of the central canal). Tracer also deposited around ependymal nuclei (marked by left arrowhead in c). The up arrow head in e points to a serpiginous trail of tracer, suggestive of privileged pathways between the subependymal vasculature and the central canal. Smooth muscle cells were labelled with SMA, endothelial cells by RECA-1