Fig. 8

Reactive oligodendrocyte progenitor cells (OPCs) in the white matter of hydrocephalic hyh mice. a–d Expression of the NG2 antigen in OPCs in neocortical frontal sections of normal (wt) and hyh mice. Immunofluorescence for NG2 (green) and bromodeoxyuridine (BrdU, red) in frontal sections of wt (a, 42 µm Z projection; c, 1 µm plane) and hyh (b, 31 µm Z projection; d, 1 µm plane) mice. The white and yellow arrows in a indicate NG2 cells in the grey and white matter, respectively. NG2 cells presenting BrdU labelled nuclei (arrows in c and d) and lacking BrdU labelling (arrowheads in c and d) can be observed (details in insets). Pericytes (yellow asterisk in b) also exhibited NG2 labelling. The lines in a and b indicate the border between white and grey matter. In wt mice proliferating neuroblasts in the subventricular zone presented BrdU labelling (white asterisk in c). e Intensity of NG2 immunoreactivity (AU, arbitrary units) in the white matter of wt ( n = 4) and hyh (n = 4) mice. f, g Density of OPCs labelled with NG2 in the white matter of wt and hyh mice without (f) (wt, n = 4; hyh n = 4) and after (g) (wt, n = 4; hyh n = 5) a pulse of BrdU. *p < 0.05; the Wilcoxon-Mann–Whitney test. gm grey matter, v lateral ventricle lumen, wm white matter