Fig. 4

Analysis of lipids by MALDI-MSI showing differential expression between wt and hydrocephalic hyh mice. Ion images of diacylglycerophosphoserine PS (42:9), diacylglycerophosphoinositol PI (38:5), and PI (38:4) in neocortical (ncx) frontal sections of wt (a, e, i) and hyh (b, f, j) mice obtained in negative ion mode at a spatial resolution of 80 μm. Images of frontal sections in pairs: left image: rostral neocortex (anterior commissure level); right image: caudal neocortex adjacent to hippocampus (hip). The white (wm) and grey (gm) matter are labelled. The heat bar represents the relative intensity, indicating the abundance of each ion. The averaged monoisotopic peak for each lipid (c, g, k) and the quantification of these peaks are shown (d, h, l). *p < 0.05, **p < 0.005, ***p < 0.0005; Student’s t-test (wt, n = 3; hyh n = 3)