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Fig. 1 | Fluids and Barriers of the CNS

Fig. 1

From: Comparative assessment of in vitro BBB tight junction integrity following exposure to cigarette smoke and e-cigarette vapor: a quantitative evaluation of the protective effects of metformin using small-molecular-weight paracellular markers

Fig. 1

Side-by-side assessment of TS/EC-induced barrier disruption and protective effect of metformin. A MTT assay of tested treatments shows no significant impact of TSe, ECe, and MF on cell viability. B TSe and ECe negatively impact BBB integrity, as demonstrated by TEER measurement. Pre-treatment with metformin at either 10 or 20 µM concentration abrogated the impact of TSe and ECe on the barrier's integrity. (*p < 0.05; ***p < 0.001; ****p < 0.0001 vs. control; +++p < 0.001 vs. 6 h. ANOVA; n = 3 biological replicates)

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Fluids and Barriers of the CNS

ISSN: 2045-8118