Fig.2
From: Brain pharmacokinetics of two BBB penetrating bispecific antibodies of different size
LigandTracer-based real-time kinetics of [125I]mAb3D6-scFv8D3 (red) and [125I]di-scFv3D6-8D3 (blue) binding to mTfR1. Recording included 10 min of baseline, 3 h of association phase 1 with 2 nM of 125I-labeled antibody, 4 h of association phase 2 with 6 nM of 125I-labeled antibody, and a dissociation phase overnight. The bold line represents the fitted curve from a 1:1 binding model for [125I]mAb3D6-scFv8D3 and a 1:2 binding model for [125I]di-scFv3D6-8D3