Fig. 2

Confirmation of HIF-1α deletion in primary astrocytes. a Micrographs showing primary rat astrocytes immunostained for GLAST, GFAP, Iba1, CD31, PDGFRβ and NeuN expression, counterstained with DAPI (blue). Scale bar = 200 nm. b MTT conversion of primary AC after 24 h normoxic or hypoxic exposure with HIF-1 KD (siHIF1α) or untreated (UNT) and scrambled controls (scram). c Representative Western blot and d quantification of HIF-1α KD efficiency in primary ACs after 6 h hypoxia. Messenger RNA levels of HIF-1 target genes Glut1, CA9 and VEGF-A in primary KD ACs compared to normoxic and hypoxic controls after 24 h hypoxia (e–g). Students t-test and 2way ANOVA, mean ± SD, n = 4–6, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 compared to Nx UNT, ^#p < 0.05, ^##p < 0.01 ^####p < 0.0001 to Hx UNT, ^$p < 0.05, ^$$$p < 0.001 compared to Hx scram