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Fig. 2 | Fluids and Barriers of the CNS

Fig. 2

From: Protecting P-glycoprotein at the blood–brain barrier from degradation in an Alzheimer’s disease mouse model

Fig. 2

Effect of NCZ on P-gp-Mediated hAβ42 transport. a Representative confocal images of isolated brain capillaries showing accumulation of fluorescein-hAβ42 in capillary lumens after a 1 hour incubation (steady state; 5 µM fluorescein-hAβ42). b Data after digital image analysis using ImageJ. Specific fluorescein-hAβ42 fluorescence is the difference between total luminal fluorescein-hAβ42 fluorescence and fluorescein-hAβ42 fluorescence in the presence of the P-gp-specific inhibitor PSC833 (5 µM). Statistics: Data per group are given as mean ± S.E.M. for 10 capillaries from one preparation (pooled tissue: WT (10 mice), hAPP (15 mice), hAPP-NCZ (15 mice), hAPP-NCZ/CSA (15 mice)). Shown are arbitrary fluorescence units (scale 0–255). ***, significantly lower than control, p < 0.001

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Fluids and Barriers of the CNS

ISSN: 2045-8118