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Fig. 5 | Fluids and Barriers of the CNS

Fig. 5

From: Chemically defined human vascular laminins for biologically relevant culture of hiPSC-derived brain microvascular endothelial cells

Fig. 5

iBMECs exhibit a more activated phenotype on CN IV-FN compared to LN 511-E8. ac Fold change in gene expression level for ANGPT1, ANGPT2, and VWF (a), various MMPs (b), and ECM proteins (c) for iBMECs on LN 511-E8 relative to iBMECs on CN IV-FN. * indicates P < 0.0005, ** indicates P < 0.001, # indicates P < 0.05, and ## indicates P < 0.01 via unpaired t-test compared to CN IV-FN for each gene. d, e Wound healing assay to assess migration of iBMECs on LN 511-E8 and CN IV-FN. d Representative images of the cells immediately after and 9 h after the ibidi wound healing culture inserts were removed from the plates. Cell-free area is outlined in red. Scale bars indicate 500 μm. e Quantification of percentage of the initial wound area that has closed after 9 h for each condition. # indicates P < 0.05 compared to CN IV-FN

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