Fig. 3
From: Preterm intraventricular hemorrhage in vitro: modeling the cytopathology of the ventricular zone
Cell junction impairments in the IVH in vitro model. a, b Comparisons of the expression of N-Cad at day 7 after 48 h of control (a) or blood (b). a Shows higher intensity of N-Cad expression and b a clear lack of N-Cad expression. a′, b′ High magnification (×63) of a, b in which a′ shows the typical lattice-like configuration of N-Cad vs b′ that shows fragmentation (arrowhead), dislocation (asterisk) and diminution of the expression of N-Cad. c N-cad protein levels quantified by WB normalized with GADPH after 48 h of treatment; blood significantly decreases the expression of N-Cad (P < 0.05). Data are mean values from relative units (n = 8). Scale bars: a, b = 100 μm; a′, b′ = 20 μm