Differential expression of receptors mediating receptor-mediated transcytosis (RMT) in brain microvessels, brain parenchyma and peripheral tissues of the mouse and the human

Table 1 Expression levels of genes encoding RMT receptors in isolated human brain microvessels (BMV), brain and lung (RNAseq normalized read counts; average ± SD)

Receptors/proteins	Human BMV	Human brain	Human lung
TFRC	1645.22 ± 249.68	1560.20 ± 520.04	7856.4 ± 6648.12
INSR	3010.84 ± 1158.90*	854.14 ± 81.00	1260.72 ± 106.44
IGF1R	1304.87 ± 746.61	905.46 ± 229.11	800.57 ± 198.75
IGF2R	602.08 ± 550.37	613.27 ± 194.15	4162.89 ± 2237.01*
LRP1	6896.83 ± 2520.15	5797.91 ± 1220.33	12,733.83 ± 4968.54
LDLR	1424.41 ± 2003.73*	294.13 ± 121.19	6952.95 ± 2791.13
LRP8 (ApoER2)	695.27 ± 650.56	734.55 ± 153.52	116.66 ± 69.72
CDC50A/TMEM30A	5578.82 ± 1174.14	7977.31 ± 2267.54	7621.38 ± 3189.82
SLC2A1/GLUT1	9579.89 ± 7741.88^#	3110.7 ± 1033.78	472.20 ± 217.86
SLC3A2/CD98hc	6527.14 ± 3983.38	4619.39 ± 509.35	5088.29 ± 425.63
LEPR	1131.17 ± 617.91*	1932.75 ± 612.91	3549.49 ± 132.05

Comparison of gene expression (transcript abundance) across isolated human BMVs, brain and lung was performed using One-way ANOVA, followed by Tukey’s multiple comparisons test. Significant difference was indicated by (*). For simplicity, statistical significance was shown only for comparisons of BMV against other tissues (but not among other tissues)
*INSR expression in BMV was significantly (p < 0.05) higher compared to either brain or lung
*IGF2R expression in lung tissue was significantly (p < 0.05) higher compared to either brain vessels or brain
*LDLR expression in BMV was significantly higher (p < 0.001) compared to brain and significantly lower (p < 0.05) compared to lung
^#SLC2A1/GLUT1: one sample out of 3 showed low transcript level, causing high SD. Therefore, despite overall high abundance, there was no significant difference compared to brain and lung. *LEPR expression in BMV was significantly lower compared to lung (p < 0.001)

ISSN: 2045-8118