Table 6 Top regulated soloculture enriched pathways at 24 h (top), 48 h (middle) and 96 h (bottom) for (A) the comparison of soloculture vs. coculture with human pericytes (CHP); and for (B) the comparison of soloculture vs. coculture with bovine pericytes (CBP)
A. | B. | ||||
|---|---|---|---|---|---|
24 h | 24 h | ||||
Pathway | Ratio | p-value | Pathway | Ratio | p-value |
Post-Golgi vesicle mediated transport | 1.7 | 1.10E−06 | Regulation of cell morphogenesis involved in differentiation | 2.1 | 6.90E−04 |
Regulation of programmed cell death | 1.7 | 6.50E−06 | Salivary gland morphogenesis | 3.0 | 7.60E−04 |
Regulation of cell death | 1.7 | 8.70E−06 | Salivary gland development | 3.0 | 1.44E−03 |
Regulation of apoptotic process | 1.7 | 1.40E−05 | Lysosomal transport | 2.0 | 1.72E−03 |
Golgi vesicle transport | 1.8 | 1.90E−05 | Establishment of protein localization to plasma membrane | 3.3 | 2.66E−03 |
Protein folding | 1.7 | 4.10E−05 | Protein localization to plasma membrane | 2.0 | 2.94E−03 |
Regulation of transcription from RNA polymerase II promotor in response to hypoxia | 3.8 | 4.60E−05 | Positive regulation of osteoblast proliferation | 2.0 | 3.27E−03 |
Negative regulation of cell death | 1.9 | 5.40E−05 | Plasma membrane organization | 2.0 | 4.00E−03 |
Negative regulation of programmed cell death | 1.9 | 5.70E−05 | Toll-like receptor 3 signaling pathway | 1.6 | 5.24E−03 |
De novo protein folding | 2.0 | 6.60E−05 | Exocrine system development | 3.0 | 5.72E−03 |
48 h | 48 h | ||||
RNA export from nucleus | 1.5 | 2.30E−05 | RNA splicing, via transesterification reactions | 4.3 | 9.20E−08 |
mRNA export from nucleus | 1.6 | 7.00E−05 | mRNA splicing, via spliceosome | 4.1 | 2.70E−07 |
Post-Golgi vesicle-mediated transport | 1.5 | 3.40E−04 | RNA splicing, via transesterification reactions with bulged adenosine | 4.1 | 2.70E−07 |
Protein localization to endosome | 7.0 | 5.80E−04 | Regulation of cellular protein catabolic process | 1.8 | 4.00E−07 |
Type I interferon production | 1.8 | 7.00E−04 | Regulation of proteolysis involved in cellular protein catabolic process | 1.9 | 5.80E−07 |
Regulation of type I interferon production | 1.8 | 7.00E−04 | Positive regulation of cellular protein catabolic process | 2.5 | 1.70E−06 |
Regulation of monocyte differentiation | 2.0 | 7.60E−04 | mRNA metabolic process | 2.2 | 2.50E−06 |
Golgi localization | 2.0 | 7.60E−04 | Ribonucleoside triphosphate biosynthetic process | 1.6 | 2.80E−06 |
Protein K48-linked ubiquitination | 1.6 | 1.00E−03 | Positive regulation of proteolysis involved in cellular protein catabolic process | 2.7 | 3.40E−06 |
Pyrimidine nucleotide metabolic process | 1.8 | 1.46E−03 | mRNA processing | 2.6 | 4.20E−06 |
96 h | 96 h | ||||
Arp2/3 complex-mediated actin nucleation | 2.0 | 7.50E−04 | Positive regulation of protein ubiquitination | 2.8 | 1.00E−05 |
Pyrimidine nucleoside triphosphate metabolic process | 1.8 | 1.01E−03 | Positive regulation of ligase activity | 3.9 | 1.90E−05 |
Programmed necrotic cell death | 4.5 | 1.88E−03 | Positive regulation of protein modification by small protein conjugation or removal | 2.6 | 2.00E−05 |
Necroptotic process | 9.0 | 2.59E−03 | Nucleoside monophosphate metabolic process | 2.2 | 2.70E−05 |
Sprouting angiogenesis | 2.0 | 3.53E−03 | Positive regulation of ubiquitin-protein transferase activity | 4.3 | 4.40E−05 |
Regulation of necroptotic process | 5.0 | 3.74E−03 | Regulation of protein modification by small protein conjugation or removal | 2.1 | 8.00E−05 |
Fibroblast apoptotic process | 2.0 | 3.74E−03 | Regulation of protein ubiquitination | 2.1 | 8.60E−05 |
Deoxyribonucleotide biosynthetic process | 4.0 | 4.17E−03 | Ribonucleoside monophosphate metabolic process | 1.9 | 1.10E−04 |
Positive regulation of protein catabolic process | 2.0 | 5.23E−03 | Regulation of ligase activity | 4.0 | 2.30E−04 |
Positive regulation of protein modification by small protein conjugation or removal | 1.8 | 5.81E−03 | Positive regulation of execution phase of apoptosis | 4.0 | 3.50E−04 |