Contribution of brain pericytes in blood–brain barrier formation and maintenance: a transcriptomic study of cocultured human endothelial cells derived from hematopoietic stem cells

Table 11 Top differentially expressed pathways for the comparison of soloculture vs. coculture with human pericytes (CHP) at 96 h

Term	Database	Corrected p-value
RHO GTPase effectors	Reactome	1.02E−04
DNA methylation	Reactome	0.004
SIRT1 negatively regulates rRNA expression	Reactome	0.005
Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3	Reactome	0.005
RHO GTPases activate formins	Reactome	0.008
RNA polymerase I promoter opening	Reactome	0.009
B-WICH complex positively regulates rRNA expression	Reactome	0.010
RHO GTPases activate PKNs	Reactome	0.0131
Signal Transduction	Reactome	0.022
Formation of the beta-catenin/TCF transactivating complex	Reactome	0.024
G1/S-specific transcription	Reactome	0.026
Mineral absorption	KEGG	0.032
Epigenetic regulation of gene expression	Reactome	0.038
Extracellular matrix organization	Reactome	0.041
Regulation of insulin-like growth factor (IGF) transport and uptake by insulin-like growth factor binding proteins (IGFBPs)	Reactome	0.046

Pathway analysis was conducted using different databases i.e. KEGG pathway, Reactome and Panther. Statistical analysis was performed using the Fisher’s exact test and false discovery rate correction test was performed by the Benjamini and Hochberg method [3]

ISSN: 2045-8118