Contribution of brain pericytes in blood–brain barrier formation and maintenance: a transcriptomic study of cocultured human endothelial cells derived from hematopoietic stem cells

Table 10 Top differentially expressed pathways for the comparison of soloculture vs. coculture with bovine pericytes (CBP) at 96 h

Term	Database	Corrected p-value
Regulation of insulin-like growth factor (IGF) transport and uptake by insulin-like growth factor binding proteins (IGFBPs)	Reactome	3.31E−04
Gap junction assembly	Reactome	0.003
Gap junction trafficking	Reactome	0.008
Gap junction trafficking and regulation	Reactome	0.008
Degradation of the extracellular matrix	Reactome	0.012
Inhibition of voltage gated Ca²⁺ channels via beta/gamma subunits	Reactome	0.015
Activation of G protein gated potassium channels	Reactome	0.015
G protein gated potassium channels	Reactome	0.015
Cell cycle, mitotic	Reactome	0.018
Cell cycle	Reactome	0.020
Extracellular matrix organization	Reactome	0.021
Inwardly rectifying K⁺ channels	Reactome	0.027
Phenylalanine metabolism	KEGG	0.027
Deposition of new CENPA-containing nucleosomes at the centromere	Reactome	0.038
Nucleosome assembly	Reactome	0.038
Intrinsic pathway of fibrin clot formation	Reactome	0.047
GABA B receptor activation	Reactome	0.048
Activation of GABAB receptors	Reactome	0.048

Pathway analysis was conducted using different databases i.e. KEGG pathway, Reactome and Panther. Statistical analysis was performed using the Fisher’s exact test and false discovery rate correction test was performed by the Benjamini and Hochberg method [3]

ISSN: 2045-8118