Table 2 Selected molecular markers of relevant CNS cell types with species and methodological information
From: Advancing brain barriers RNA sequencing: guidelines from experimental design to publication
Endothelial cells of the BBB | Pericytes | Astrocytes | Smooth muscle cells | CP/CVO endothelial cells | CP epithelial cells | Immune cells | Oligodendrocytes | Fibroblasts | |
|---|---|---|---|---|---|---|---|---|---|
AQ1 | – | – | – | – | – | [114] IHC [115] IHC | – | – | – |
AQP4 | – | – | [116] IHC | – | – | [114] IHC | – | – | – |
⍺-SMA | – | ± IHC [117] IHC [118] | – | [119] IHC | – | – | – | – | – |
CD31/PECAM1 | – | – | – | [122] IHC | – | [123] IHC | – | – | |
Cdh5 | [124] | – | – | – | [125] RM | – | – | – | – |
Cldn-1 | [126] IHC | – | – | – | – | – | – | – | |
Cldn-2 | – | – | – | – | – | – | – | – | |
Cldn-3 | [130] IF [79]a | – | – | – | – | – | – | – | – |
Cldn-5 | [131] KO + IHC/WB [132] IHC | – | – | – | – | [127] IHC | – | – | – |
Cldn-11 | – | – | – | – | – | [128] IHC | – | IHC [133] IHC [134] | – |
FGF | WB [135] | ||||||||
GFAP | – | – | [136] IF | – | – | – | – | – | – |
GLAST | – | – | WB [137] | – | – | – | – | – | – |
ICAM-1 | [138] IHC [139] ELISA | [140] ICC | – | – | – | – | [141] | – | – |
MHC-II | – | – | – | – | – | – | [142] PCR | – | – |
NG2/CSPG4 | – | [143] IHC | – | – | – | – | [144] Immunogold | – | |
OAP-1 | – | – | – | – | – | – | – | [134] WB + IHC | – |
Occludin | [132] IHC | – | – | – | [127] IHC | – | – | – | |
PDGFR-β | – | [143] IHC | – | – | – | – | – | – | – |
Podoplanin | – | – | – | – | – | [145] IHC, WB [146] IHC | – | – | – |
TTR | – | – | – | – | – | [41] QPCR, RNAseq | – | – | – |
VCAM-1 | [147] ELISA | [140] ICC | – | – | – | – | – | – | – |
ZO-1 | [148] IHC [149] ICC | – | – | – | – | [128] IHC | – | – | – |