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Table 2 Selected molecular markers of relevant CNS cell types with species and methodological information

From: Advancing brain barriers RNA sequencing: guidelines from experimental design to publication

  Endothelial cells of the BBB Pericytes Astrocytes Smooth muscle cells CP/CVO endothelial cells CP epithelial cells Immune cells Oligodendrocytes Fibroblasts
AQ1 [114] IHC
[115] IHC
AQP4 [116] IHC [114] IHC
⍺-SMA ± IHC [117]
IHC [118]
[119] IHC
CD31/PECAM1 [120, 121] IHC [122] IHC [123] IHC
Cdh5 [124] [125] RM
Cldn-1 [126] IHC [127,128,129] IHC
Cldn-2 [127, 128] IHC
Cldn-3 [130] IF
[79]a
Cldn-5 [131] KO + IHC/WB
[132] IHC
[127] IHC
Cldn-11 [128] IHC IHC [133]
IHC [134]
FGF          WB [135]
GFAP [136] IF
GLAST WB [137]
ICAM-1 [138] IHC
[139] ELISA
[140] ICC [141]
MHC-II [142] PCR
NG2/CSPG4 [143] IHC   [144] Immunogold
OAP-1 [134] WB + IHC
Occludin [132] IHC [127] IHC [127, 128] IHC
PDGFR-β [143] IHC
Podoplanin [145] IHC, WB
[146] IHC
TTR [41] QPCR, RNAseq
VCAM-1 [147] ELISA [140] ICC
ZO-1 [148] IHC
[149] ICC
[128] IHC
  1. Italic represents evidence in the mouse, bold italic represents evidence in humans, underline represents evidence in the mouse and humans
  2. IHC immunohistochemistry, RM reporter mice, IF immunofluorescence, PCR polymerase-chain reaction, QPCR quantitative polymerase-chain reaction, RNASeq next generation RNA sequencing, scRNAseq single-cell RNA sequencing, KO knock-out, WB Western-blot, ICC immunocytochemical staining, ELISA enzyme-linked immunosorbent assay
  3. aA study showing contrary evidence (absence)