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Fig. 1 | Fluids and Barriers of the CNS

Fig. 1

From: Inhibition of transforming growth factor beta signaling pathway promotes differentiation of human induced pluripotent stem cell-derived brain microvascular endothelial-like cells

Fig. 1

The effect of TGF-β inhibitor on iBMELCs differentiation. a A schematic diagram of the protocol of differentiation of human iPSCs to BMECs. b Immunofluorescence for the endothelial cell adhesion molecule (VE-cadherin: red). Blue: DAPI. Scale bar, 100 μm. Statistical significance was calculated using the unpaired Student’s t-test (**p < 0.01), control = 1. Data are represented as the mean ± SD (n = 3). c Ac-LDL (red) uptake assay. Blue: Hoechst 33342. Scale bar, 100 μm. Positive cell ratio was defined as the number of cells with Ac-LDL uptake to the total number of cells. Statistical significance was calculated using the unpaired Student’s t-test (***p < 0.001). Data are represented as the mean ± SD (n = 3). d Tube formation assay. The cells were stained with calcein (green). Scale bar, 500 μm

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Fluids and Barriers of the CNS

ISSN: 2045-8118