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Fig. 1 | Fluids and Barriers of the CNS

Fig. 1

From: Culture-induced changes in mRNA expression levels of efflux and SLC-transporters in brain endothelial cells

Fig. 1

BBB markers. a Schematic drawing of the different in vitro culture configurations. Bovine capillary endothelial cells were seeded on the apical side of the filter in all culture configurations. In non-contact co-culture, the rat astrocytes were seeded in the bottom of the well, whereas they were seeded on the basolateral side of the filter in the contact co-culture. b Quantitative analysis of the mRNA expression levels of VWF. The expression of VWF were significantly higher in NCC and CCC compared to capillaries. c Quantitative analysis of the mRNA expression levels of ALP. There were no significant difference in the expression levels. d Quantitative analysis of the mRNA expression levels of TPA. All culture configurations were significantly downregulated compared to capillaries. MC mono culture, NCC non-contact co-culture and CCC contact co-culture, vWF von Willebrand Factor, ALP alkaline phosphatase, TPA tissue plasminogen activator. n = 5 − 3, N = 3. **: P ≤ 0.005. Error bars are Mean + SEM

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Fluids and Barriers of the CNS

ISSN: 2045-8118