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Fig. 2 | Fluids and Barriers of the CNS

Fig. 2

From: Recent advances in human iPSC-derived models of the blood–brain barrier

Fig. 2

Schematic of differentiation protocols for deriving brain microvascular endothelial cells from induced pluripotent stem cells and main assay readouts for assessing BMEC phenotype. Main advancements from previous protocols are bolded. bFGF basic fibroblast growth factor, MEF mouse embryonic fibroblast, KOSR knockout serum replacement, l-glutl-glutamine, β-ME β-mercaptoethanol, ECSFM endothelial cell serum free media, PDS platelet-poor plasma derived serum, VEGF vascular endothelial growth factor, TEER transendothelial electrical resistance, ICC immunocytochemistry, ETA efflux transporter activity, FC flow cytometry, TEM transmission electron microscopy, RT-PCR reverse transcription polymerase chain reaction, qPCR quantitative polymerase chain reaction

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Fluids and Barriers of the CNS

ISSN: 2045-8118