Table 1 RT-qPCR primer sequences
Genes | Forward primer sequence (5′ → 3′) | Reverse primer sequence (5′ → 3′) |
|---|---|---|
CDH5 | GATTTGGAACCAGATGCACA | ACTTGGCATTCTTGCGACTC |
MDR1 | CCCATCATTGCAATAGCAGG | TGTTCAAACTTCTGCTCCTGA |
BCRP | AGATGGGTTTCCAAGCGTTCAT | CCAGTCCCAGTACGACTGTGACA |
GLUT1 | GAAGAGAGTCGGCAGATGATG | GGAGTAATAGAAGACAGCGTTGATG |
Occludin | TCCAATGGCAAAGTGAATGA | GCAGGTGCTCTTTTTGAAGG |
ZO-1 | CGAGGGATAGAAGTGCAAGTAGA | TATTCTTCATTTTTCCGGGATTT |
LAT1 | AATGGGTCCCTGTTCACATC | CGTAGAGCAGCGTCATCACA |
HPRT1 | CTTTGCTTTCCTTGGTCAGG | TCAAGGGCATATCCTACAACA |
PECAM1 | AGTCGGACAGTGGGACGTAT | ATGACCTCAAACTGGGCATC |
vWF | CGGCTTGCACCATTCAGCTA | TGCAGAAGTGAGTATCACAGCCATC |
KDR | CTGCAAATTTGGAAACCTGTC | GAGCTCTGGCTACTGGTGATG |
TBXT | ACCCAGTTCATAGCGGTGAC | CAATTGTCATGGGATTGCAG |
KDR | CTGCAAATTTGGAAACCTGTC | GAGCTCTGGCTACTGGTGATG |
OCT-4 | AGCGAACCAGTATCGAGAAC | TTACAGAACCACACTCGGAC |
NANOG | CTGCAGAGAAGAGTGTCGCA | ACCAGGTCTTCACCTGTTTG |