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Table 1 Fluorophore labelled antibodies and isotype controls for FACS analysis

From: Human CD4+ T cell subsets differ in their abilities to cross endothelial and epithelial brain barriers in vitro

Antibodies

Fluorophore

Clone

Source

Cat. N.

Isotype

Anti-human ICAM1

BV421

HA58

BD Biosciences

564077

m-IgG1, κ

Anti-human ICAM2

PE

CBR-IC2/2

BD Biosciences

558080

m-IgG2a, κ

Anti-human VCAM1

FITC

51-10C9

BD Biosciences

551146

m-IgG1, κ

Anti-human P-selectin

BV510

AC1.2

BD Biosciences

743756

m-IgG1, κ

Anti-human E-selectin

APC

68-5H11

BD Biosciences

551144

m-IgG1, κ

Anti-human CD99

PE-Cy7

3B2/TA8

Biolegend

371314

m-IgG2a, κ

Isotype controls

Fluorophore

Clone

Source

Cat. N.

 

m-IgG1, κ

APC

MOPC-21

BD Biosciences

555751

 

m-IgG1, κ

BV421

X40

BD Biosciences

562438

 

m-IgG2a, κ

PE

eBM2a

Invitrogen

12-4724-42

 

m-IgG1, κ

FITC

MOPC-21

Biolegend

400108

 

m-IgG1, κ

BV510

X40

BD Biosciences

562946

 

m-IgG2a, κ

PE-Cy7

MOPC-173

BIolegend

400232

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