Fig. 5

CD4⁺ T-cell migration across HIBCPP from CSF side to choroid plexus stroma side. a CD4⁺ T-cell (Th1, Th1*, Th2 and Th17) migration rate across non-stimulated (NS) or 16 h pro-inflammatory cytokine-stimulated (1 ng/mL TNF-α + 20 IU/mL IFN-γ) standard HIBCPP cell monolayers was measured after 8 h transmigration assay. Percentages of transmigrated T cells from peripheral blood of two healthy donors (Donor B and C) across standard HIBCPP cell monolayer are displayed. Data are shown as the mean on superimposed scatter dot plot of 4 independent experiments each performed in triplicates. Statistical analysis: two-way ANOVA followed by Tukey’s multiple comparison test within conditions (between subsets) (p < 0.05*, p < 0.01**, p < 0.001***, p < 0.0001****). b Percentage of transmigrated T-cells from peripheral blood of healthy donors B and C across 16 h pro-inflammatory cytokine-stimulated (1 ng/mL TNF-α + 20 IU/mL IFN-γ) standard HIBCPP cells monolayer pretreated with either anti-human ICAM-1 blocking antibody (10 μg/mL) or isotype control antibody are shown. CD4⁺ T-cells (Th1, Th1*, Th2 and Th17) were allowed to migrate across standard HIBCPP cells monolayer for 8 h and migrated cells were collected and counted. Results are standardized to isotype control (100%). Data are shown as the mean on superimposed scatter dot plot of 4 independent experiments each performed in triplicates. Statistical analysis: two-way ANOVA followed by Tukey’s multiple comparison test. (p < 0.0001****). Cells used for the representation of the endothelium, the epithelium (HIBCPP cells) and T cells are adapted from Servier Medical Art (http://smart.servier.com/), licensed under a Creative Common Attribution 3.0 Generic License