Fig. 2

Analysis of plasma membrane transporter expression. Immunoblot analysis of the protein abundance in the CP from Ncbe wt and Ncbe ko mice for a Ncbe, b AQP1, c Na,K-ATPase β1 subunit, d Proteasome 20 s, e Na,K-ATPase α1 subunit, f NKCC1, g Ae2, and h Proteasome 20 s. Immunofluorescence histochemistry was applied to compare protein expression of NKCC1 (green) in the IVth ventricle CP from i Ncbe wt and j Ncbe ko mice. Arrows indicate the luminal plasma membrane, while arrowheads indicate the basolateral membrane labyrinth. Nuclei are stained blue. k Scatter plot comparing the relative changes in transporter protein abundance obtained by immunofluorescence microscopy (IF), proteomic mass spectrometry analysis (MS), and immunoblotting (IB) (*p < 0.05, n = 5). Mean values are normalized to control (Ncbe wt) and indicated by horizontal bars. Triangles indicate data points from Ncbe wt CP, whereas circles represent data from Ncbe ko CP. Mean data for the IF semi-quantitation, except for NKCC1 are from previous publications [13,14,15]