Skip to main content

Table 1 Primary and secondary antibodies used for protein expression studies

From: Region-specific blood–brain barrier transporter changes leads to increased sensitivity to amisulpride in Alzheimer’s disease

ProteinPrimary antibodySecondary antibody Western blot (WB)
OCT-1 (SLC22A1)Rabbit polyclonal anti-human and mouse (Abcam, Cat#ab55916; RRID:AB_882579), WB dilution—1:250Goat anti-rabbit HRP (Abcam, cat#ab6721: RRID:AB_955447) dilution—1:1000
OCT-2 (SLC22A2)Rabbit monoclonal to human and mouse (Abcam, Cat#ab170871: RRID:AB_2751021), WB dilution—1:2000Goat anti-rabbit HRP (Abcam, cat#ab6721: RRID:AB_955447) dilution—1:2000
OCT-3 (SLC22A3)Rabbit polyclonal to human and mouse (Abcam, Cat#ab183071; RRID:AB_2751016), WB dilution—1:600Goat anti-rabbit HRP (Abcam, cat#ab6721: RRID:AB_955447) dilution—1:2000
OCTN1 (SLC22A4)Rabbit polyclonal to human and mouse (Abcam, Cat#ab200641; RRID:AB_2751017), WB dilution—1:1000Goat anti-rabbit (IgG)-HRP (Cell Signalling. Cat#7074S:AB_2099233) dilution—1:1000
OCTN2 (SLC22A5)Rabbit polyclonal to human and mouse (Abcam, Cat#ab180757; RRID:AB_2751018), WB dilution—1:1000Goat anti-rabbit (IgG)-HRP (Cell Signalling. Cat#7074S: AB_2099233) dilution—1:1000
MATE1 (SLC47A1)Rabbit polyclonal to human from Abcam (Cat#ab104016: RRID AB_10711136), WB dilution—1:500Goat anti-rabbit HRP (Abcam, Cat#ab6721: RRID:AB_955447) dilution—1:2000
MATE1 (SLC47A1)Rabbit polyclonal to mouse from Alomone labs (Cat#ANT-131: RRID:AB_2751020), WB dilution—1:800Goat anti-rabbit HRP (Abcam, Cat#ab6721: RRID:AB_955447) dilution—1:2000
MATE2 (SLC47A2)Rabbit polyclonal to human and mouse (Abcam, Cat#ab174344: RRID:AB_2751019), WB dilution—1:500Goat anti-rabbit HRP (Abcam, Cat#ab6721: RRID:AB_955447) dilution—1:2000
PMAT (SLC29A4)Mouse monoclonal to human and rat (Abcam, Cat#ab56554: RRID:AB_2190909), WB dilution—1:500Rabbit anti-mouse HRP (Abcam, Cat#ab6728: RRID:AB_955440) dilution 1:2000
PMAT (SLC29A4)Rabbit polyclonal to human, mouse and rat (Bioss Antibodies; Cat#:bs-4176R: RRID:AB_11108960), WB Dilution—1:800 for hCMEC/D3—1:650 for b.End3, 1:600 mouse capillariesGoat anti-rabbit (IgG)-HRP (Cell Signalling. Cat#7074S: RRID:AB_2099233) dilution—1:1000
TfRTransferrin receptor monoclonal antibody (Thermo Fisher Scientific, CAT# 13-6800: RRID: AB_2533029), WB Dilution—1:1000Rabbit polyclonal Secondary to Mouse IgG—HRP (Abcam, CAT# ab6728 RRID: 955_440) WB dilution—1:1000
GAPDHRabbit polyclonal to GAPDH (Abcam, Cat#ab9485: RRID:AB_307275), WB dilution 1: 2500 or 1:10,000 
  1. The predicted antibodies with their predicted molecular weight (MW) WB were all made up in PBS-T with 5% BSA. Actual band sizes may differ due to post translational modifications or cleavages. (Validation data is available from the Abcam website (https://www.abcam.com/nav/primary-antibodies), cell signalling technology website (https://www.cellsignal.co.uk) and Alonome labs website (https://www.alomone.com). Accessed 19.10.18. Validation data is available from the BIOSS USA antibodies website (http://www.biossusaantibodies.com). Accessed 4.12.18. Verification data is available from ThermoFisherScientific (https://www.thermofisher.com). Accessed 20.11.2019