Table 1 Primary and secondary antibodies used for protein expression studies
Protein | Primary antibody | Secondary antibody Western blot (WB) |
|---|---|---|
OCT-1 (SLC22A1) | Rabbit polyclonal anti-human and mouse (Abcam, Cat#ab55916; RRID:AB_882579), WB dilution—1:250 | Goat anti-rabbit HRP (Abcam, cat#ab6721: RRID:AB_955447) dilution—1:1000 |
OCT-2 (SLC22A2) | Rabbit monoclonal to human and mouse (Abcam, Cat#ab170871: RRID:AB_2751021), WB dilution—1:2000 | Goat anti-rabbit HRP (Abcam, cat#ab6721: RRID:AB_955447) dilution—1:2000 |
OCT-3 (SLC22A3) | Rabbit polyclonal to human and mouse (Abcam, Cat#ab183071; RRID:AB_2751016), WB dilution—1:600 | Goat anti-rabbit HRP (Abcam, cat#ab6721: RRID:AB_955447) dilution—1:2000 |
OCTN1 (SLC22A4) | Rabbit polyclonal to human and mouse (Abcam, Cat#ab200641; RRID:AB_2751017), WB dilution—1:1000 | Goat anti-rabbit (IgG)-HRP (Cell Signalling. Cat#7074S:AB_2099233) dilution—1:1000 |
OCTN2 (SLC22A5) | Rabbit polyclonal to human and mouse (Abcam, Cat#ab180757; RRID:AB_2751018), WB dilution—1:1000 | Goat anti-rabbit (IgG)-HRP (Cell Signalling. Cat#7074S: AB_2099233) dilution—1:1000 |
MATE1 (SLC47A1) | Rabbit polyclonal to human from Abcam (Cat#ab104016: RRID AB_10711136), WB dilution—1:500 | Goat anti-rabbit HRP (Abcam, Cat#ab6721: RRID:AB_955447) dilution—1:2000 |
MATE1 (SLC47A1) | Rabbit polyclonal to mouse from Alomone labs (Cat#ANT-131: RRID:AB_2751020), WB dilution—1:800 | Goat anti-rabbit HRP (Abcam, Cat#ab6721: RRID:AB_955447) dilution—1:2000 |
MATE2 (SLC47A2) | Rabbit polyclonal to human and mouse (Abcam, Cat#ab174344: RRID:AB_2751019), WB dilution—1:500 | Goat anti-rabbit HRP (Abcam, Cat#ab6721: RRID:AB_955447) dilution—1:2000 |
PMAT (SLC29A4) | Mouse monoclonal to human and rat (Abcam, Cat#ab56554: RRID:AB_2190909), WB dilution—1:500 | Rabbit anti-mouse HRP (Abcam, Cat#ab6728: RRID:AB_955440) dilution 1:2000 |
PMAT (SLC29A4) | Rabbit polyclonal to human, mouse and rat (Bioss Antibodies; Cat#:bs-4176R: RRID:AB_11108960), WB Dilution—1:800 for hCMEC/D3—1:650 for b.End3, 1:600 mouse capillaries | Goat anti-rabbit (IgG)-HRP (Cell Signalling. Cat#7074S: RRID:AB_2099233) dilution—1:1000 |
TfR | Transferrin receptor monoclonal antibody (Thermo Fisher Scientific, CAT# 13-6800: RRID: AB_2533029), WB Dilution—1:1000 | Rabbit polyclonal Secondary to Mouse IgG—HRP (Abcam, CAT# ab6728 RRID: 955_440) WB dilution—1:1000 |
GAPDH | Rabbit polyclonal to GAPDH (Abcam, Cat#ab9485: RRID:AB_307275), WB dilution 1: 2500 or 1:10,000 |