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Fig. 6 | Fluids and Barriers of the CNS

Fig. 6

From: Enhanced xeno-free differentiation of hiPSC-derived astroglia applied in a blood–brain barrier model

Fig. 6

In vitro modelling of the blood–brain barrier (BBB) using hiPSC-derived brain endothelial cells (r-iBECs) cocultured with hiPSC-derived astroglia differentiated on human, recombinant laminin-521 (LN521) or murine laminin (L2020). a Paracellular permeability as measured by TEER showed that endothelial cells have higher TEER in coculture with both astroglia differentiated on LN521 and L2020 as compared to monoculture. b Permeability of fluorescein was decreased in r-iBECs cocultured with both astroglia differentiated on LN521 and L2020 compared to monoculture. In addition, fluorescein permeability was lower in r-iBECs in coculture with astroglia differentiated on L2020 compared to LN521. ck Characterization of r-iBECs by mRNA expression levels of BBB associated genes in monoculture or coculture with astroglia. mRNA levels of tight junction protein 3 (TJP3) (c), adherence junction protein VE-cadherin (d), endothelial marker vWF (e), adherence junction protein CD31 (f), glucose transporter SLC1A2 (g), efflux transporter ABCG2 (h), tight junction protein 1 (TJP1) (i) and efflux transporters ABCB1 (k) were analyzed. Expression levels of VE-cadherin mRNA were observed to be higher in endothelial cells cocultured with astroglia differentiated on LN521 compared to L2020 for all lines. mRNA levels of vWF were higher in r-iBECs cocultured with astroglia derived from the C9 and AF lines differentiated on LN521 compared to differentiation on L2020. NES included as reference. Significance in Significance indicated by *p < 0.05, **p < 0.01, ***p < 0.001

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