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Fig. 4 | Fluids and Barriers of the CNS

Fig. 4

From: Enhanced xeno-free differentiation of hiPSC-derived astroglia applied in a blood–brain barrier model

Fig. 4

Glutamate metabolism related mRNA expression in hiPSC astroglia differentiated on human, recombinant laminin-521 (LN521) or murine laminin (L2020). a A schematic overview of glutamate uptake, conversion to glutamine and glutamine export in astroglia. bh mRNA expression of glutamine exporters, SNAT3 (b), SNAT5 (c), ASCT2 (d), glutamine synthase (GLUL) (e), glutamate importers EAAT1 (f) and EAAT2 (g), GABA importer SLC6A1 (h). mRNA expression of SNAT3 and EAAT1 was higher in astroglia differentiated on LN521 compared to L2020, for all three hiPSC lines. The C1 line differentiated on LN521 additionally showed higher expression of SNAT5, ASCT2 and SLC6A1 compared to C1 differentiated on L2020. Significance indicated by *p < 0.05, **p < 0.01, ***p < 0.001

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