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Fig. 2 | Fluids and Barriers of the CNS

Fig. 2

From: Enhanced xeno-free differentiation of hiPSC-derived astroglia applied in a blood–brain barrier model

Fig. 2

Characterization of proliferation and mRNA expression of astroglial marker mRNA in hiPSC astroglia differentiated on recombinant human laminin-521 (LN521) and murine laminin (L2020). a Proliferation rate analysis showed no differences between astroglia differentiated on LN521 and L2020. bi Expression analysis of astroglia related mRNA, GFAP (b), S100B (c), Nestin (d), CD44 (e), FABP7 (f), aldehyde dehydrogenase (ALDH1L1) (g), glial derived neurotrophic factor (GDNF) (h) and Angiopoietin-1 (Ang-1) (i). mRNA expression of GFAP, S100B, ALDH1L1, GDNF and Ang-1 were higher in astroglia differentiated on LN521 compared to astroglia differentiated on L2020 for all lines. The C1 and C9 lines differentiated on LN521 had higher expression of FABP7 compared to differentiation on L2020. NES included as reference. Significance indicated by *p < 0.05, **p < 0.01 ***p < 0.001

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